Which of the following enzymes is not an endogenous redox buffering system?
Select one:
a. Catalase
b. Glutathione peroxidase
c. Hexokinase
d. Superoxide dismutase
Question 2
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Which of the following vitamins is an antioxidant?
Select one:
a. B1
b. B9
c. D
d. E
e. K
Question 3
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Which of the following minerals is required for optimum activity of glutathione peroxidase?
Select one:
a. Cobalt
b. Iron
c. Magnesium
d. Selenium
e. Sodium
Question 4
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What type of antioxidants did the Outback Rejuvenating Water claim to contain?
Select one:
a. Antioxidant minerals
b. Flavonoid antioxidants
c. Kakadu plums
d. Phytochemicals
e. Triple antioxidants
Question 5
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Which vitamin is normally responsible for the reduction of ferric ion to ferrous ion in vivo?
Select one:
a. A
b. B1
c. C
d. D
e. K
Question 6
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How much of solutions i., ii., and iii. would the students require to make a working solution with a final volume of 120ml?
Select one:
a. 10ml of solution i.; 100ml of solution ii.; and 10ml of solution iii.
b. 10ml of solution i.; 10ml of solution ii.; and 100ml of solution iii.
c. 100ml of solution i.; 10ml of solution ii.; and 10ml of solution iii.
d. 10ml of solution i.; 10ml of solution ii.; and 10ml of solution iii.
e. 10ml of solution i.; 100ml of solution ii.; and 1000ml of solution iii.
Question 7
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In table one, what will be the concentration of FeSO4.7H2O in standard no. 4 (std no. 4)?
Select one:
a. 1mM
b. 0.5mM
c. 0.25mM
d. 0.125mM
e. 0.063mM
Question 8
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In table one, from which tube would you take 500μl from in order to prepare standard no. 5 (Std no. 5)?
Select one:
a. Tube 1
b. Tube 2
c. Tube 3
d. Tube 4
Question 9
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In table one, how much water should be added to prepare standard no. 1 (Std no. 1)?
Select one:
a. 0μl
b. 250μl
c. 500μl
d. 750μl
e. 1000μl
Question 10
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What actions should the students take if reading b for any sample is greater than the absorbance measured for tube 6 (the most concentrated standard)?
Select one:
a. Further dilute the sample
b. Nothing because you can extrapolate the standard curve
c. Prepare a higher standard
d. Throw the sample away and forget about it
e. Use a more concentrated sample (e.g. 1 in 8)
Question 11
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The absorbance for reading b should be higher than reading a. What actions should the students take if reading b is less than reading a?
Select one:
a. Swap the readings around and subtract b from a
b. Take the value of b minus a to be zero
c. Throw the sample away and forget about it
d. Further dilute the sample
e. Use a more concentrated sample (e.g. 1 in 8)
Question 12
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In table three, what is the ΔAbs value for Twinings Pure Green Tea?
Select one:
a. 0.405
b. 0.416
c. 0.526
d. 0.99
e. 0.41
Question 13
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In table three, what was the concentration of Fe2+ yielded by the sample containing Jacob’s Creek Shiraz Cabernet (to three decimal places)?
Select one:
a. 0.412mM
b. 0.5mM
c. 0.53mM
d. 0.682mM
e. 1.188mM
Question 14
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In table three, what is the concentration of Fe2+ yielded by the sample containing cranberry juice (to three decimal places)?
Select one:
a. 0.127mM
b. 0.136mM
c. 0.201mM
d. 0.344mM
e. 0.89mM
Question 15
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In table three, what value should be inserted into the final column (i.e. after the correction for the dilution factor) for Nescafe Green Blend Coffee (to three decimal places)?
Select one:
a. 2.772mM
b. 4.468mM
c. 4.565mM
d. 11.532mM
e. 18.597mM
Question 16
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According to the FRAP assay, which of the samples tested was the best antioxidant?
Select one:
a. Cranberry juice
b. Glaceau vitamin water
c. Jacob’s Creek Shiraz Cabernet
d. Prune juice
e. Twinings pure green tea
Question 17
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According to the FRAP assay, which of the samples tested was the worst antioxidant?
Select one:
a. Jacob’s Creek Shiraz Cabernet
b. Nescafe Green Blend Coffee
c. Outback Spirit Rejuvenating Water
d. Prune juice
e. Twinings pure green tea
Question 18
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What do the results tell you about the claims made by Nescafe Green Blend coffee that it contains 70% more antioxidants than green tea?
Select one:
a. The claim is erroneous. According to the FRAP assay, the Nescafe Green Blend Coffee has only about half the antioxidant capacity of the Twinings Pure Green Tea
b. The claim is incorrect, although according to the FRAP assay the Nescafe Green Blend Coffee does have a slightly higher antioxidant potential
c. The claim is irrelevant. The assay warns us not to drink green tea or green coffee
d. The claim is wrong. The Twinings Pure Green Tea has three times the antioxidant capacity of the Nescafe Green Blend Coffee
e. They’re right, Nescafe Green Blend Coffee does contain 70% more antioxidants
Question 19
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What do the results tell you about the claims made by prune juice to have approximately fives times the oxygen radical absorbance capacity of cranberry juice?
Select one:
a. The claim is correct. Prune juice is far better than cranberry juice
b. The claim is erroneous. According to the FRAP assay cranberry juice has far greater antioxidant capacity than prune juice
c. The claim is incorrect. According to the FRAP assay prune juice only has about twice the antioxidant capacity of cranberry juice
d. The claim is irrelevant. Drinking juice is always preferrable to drinking water
e. The claim is neither here nor there, because cranberry and prune juice were easily the most potent antioxidants tested
Question 20
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Choose the best strategy from the following options that could be used to increase the validity of the results
Select one:
a. Increase the concentration of FeCl3.6H2O in the working solution
b. Increase the incubation time for the samples to 10 minutes
c. Increase the number of replicates measured
d. Increase the number of sample dilutions prepared
e. Increase the volume of sample used from 100 to 500µl
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