Author: chris

1. Both the chromosome and plasmid are circular pieces of DNA in a bacterial cell. Explain, in your own words, how our plasmid isolation procedure selectively purifies plasmid DNA and avoid contaminating the plasmid sample with chromosomal DNA. (3 points)

2. When we purify the plasmid DNA, we discard the white solid and keep the liquid after the first 10 min spin. After that, we add isopropanol to the sample, mix, and then centrifuge again for 10 minutes. After this second centrifugation, we discard the liquid and keep the white solid. Explain, in your own words, why these two steps are different. (4 points)
3. Suppose that on a piece of circular plasmid, the sequence 5’GGATCC appears 4 times. This sequence is recognized by the restriction enzyme called NcoI. If we expose the plasmid DNA to the enzyme in the correct condition, how many fragments of DNA will you expect to recover? (2 points)
4. In setting up the restriction enzyme digestion reaction, the protocol states that you should add the appropriate amount of buffer. Why is adding the reaction buffer important? What property/properties of the restriction enzyme makes this essential? Explain. (3 points)
5. Refer to the following image depicting the expected outcome of our restriction digestion.

Within criminal justice, researchers are often faced with a plethora of anecdotal data with little empirical support for such assertions. We have all, no doubt, heard anecdotes pertaining to gang initiation rites, killers stalking baby sitters, or corpses discovered rolled in carpets by hapless hotel maids. Criminal justice researchers depend primarily upon empirical data as the most useful tools with which to explore the nuances of the issues plaguing society. However, one can always ponder the usefulness of anecdotal data. In contemporary research, there has yet to be a consensus pertaining to the proper place and function of anecdotal data. Jacques (2014) discusses both quantitative and qualitative research methods.

 

Respond fully to the following questions:

• How would you explain the differences between the use of empirical research data from that of anecdotal data?
• How do these differences inform how criminal justice data might be evaluated and interpreted in published research findings?
• Is there a place in formal research for anecdotal data?

#2

 

Within the field of criminal justice, lie a host of intriguing issues and problems. Scholars devoted to research in the area of criminal justice utilize both qualitative and quantitative data in their quest to obtain answers to the many issues inherent in the study of crime and human behavior. It is imperative for ethical, robust, and viable research that data utilized by researchers be applied appropriately and analyzed accurately.

 

Respond to the questions posed below.

• How would you describe the ways in which research data can be applied to a particular problem in criminal justice?
• How important is the evaluation and accurate interpretation of data utilized in research analyses?

Short Paper 2

There are two basic types of project selection models: non-numeric and numeric. As a project manager, you should prepare a report that provides an overview of the selection criteria, selection models, and management processes. Your short paper should do the following:

Franklin hears about people being discriminated against at the workplace and in society but rarely experiences it first hand. Cortez, on the other hand, speaks English poorly and is often subject to derogatory comments from peers and coworkers.

Reflect on your experience with racial discrimination.

Identify examples of racial discrimination and provide examples you have personally witnessed or heard about firsthand from the following three realms:

• family
• workplace
• community

Write a brief summary that includes the following:

• Describe a racial incident pertaining to each realm in detail. Explain why you believe this is racial discrimination.
• Explain how this situation could have been handled to avoid discrimination against those belonging to another race.

Submit your response to the M2: Assignment 2 Dropbox by Week 2, Day 7. Your response should be at least two pages long. All written assignments and responses should follow APA rules for attrib

 

Review the following case scenario and complete the tasks that follow:

Student: Hannah

Age: 8

Grade: 3

Hannah is a third-grade student who transferred to Cartwright Elementary School late in the fall. Her teacher, Mrs. Pei, has noticed that she seems to struggle with many independent reading assignments. When Mrs. Pei administered the mid-year universal screening measure, she was not surprised to see that Hannah’s score had fallen below the grade-level benchmark. Consequently, Mrs. Pei decided to monitor Hannah’s reading performance once a week for seven weeks using a measure of reading fluency. The rate of growth she is expected to achieve by the end of seven weeks is 1.2. Hannah’s scores are in the graph and table below.

Compose a 500-750-word evaluation of Hannah. Incorporate the following:

1. Research and Review journal articles on response-to-intervention (RTI) activities, including data-based decision-making, determining performance level, and making tier placement decisions.
2. Using the seven weeks of progress monitoring data outlined above, calculate Hannah’s slope (change in x over the change in y) and identify the change in the number of words read correctly from beginning to end. SEE ATTACHED
3. Determine whether Hannah is responding adequately to Tier 1 instruction. Elaborate.
4. Based on your evaluation, what tier of instruction would you recommend for Hannah?
5. What do you recommend Hannah read, based on her interests and background?
6. Cite a minimum of two scholarly sources.

USESource: The IRIS Center for Training Enhancements, http://iris.peabody.vanderbilt.edu/

 

(1) First identify one or two aspects of the course that added to your knowledge of personality development, in general, and explain why.

(2) Then identify at least one thing you learned that is directly related to your own area of interest. For example, if your area of interest is criminal justice, what can you take away from this class that will help you with your future work in that area? If your area of interest is psychology, how will what you learned in this class help you be successful in future psychology courses? If your area of interest is being a better spouse and/or parent, then what new tools can you glean from this course for that purpose? If there is more than one area to which you think you can apply what you have learned, you may, of course, discuss more than one, but only one application of learning to interest area is required for this last post.

Important: You shouldn’t consider this one a “fluff” discussion. Give it thoughtful consideration. What did you learn and why was it interesting enough to make you want to discuss it here?

Your initial post of 300 words minimum

HS311-4:

 

Examine summary statistics of health data in terms of variability.

 

Calculate summary statistics of one of the variables in the sample dataset of weight and height that’s provided in Doc Sharing. Calculate the mean, median, and standard deviation. In addition provide either a five-number summary or box plot. Based on the statistics, describe the data in your own words, noting any trends that you see.

 

Discussion 1

 

Read the Forbes article “The Difference Between Coaching and Mentoring.” Coaching and mentoring are both functions of human resources management. Many of the characteristics are similar. However, the two techniques are best used for different situations. Describe the main differences between coaching and mentoring. Give an example of a scenario in which coaching is the better method. Give an example in which mentoring is the better method.

Reference and cite the textbook in your original post.

 

Discussion 2

 

Corporate social responsibility is a function of human resources (HR). However, HR must ensure that employees understand the importance of corporate social responsibility and how they are affected. Watch the video clip on Esprit and Patagonia. After viewing the clip, answer the following questions:

• How do these two organizations demonstrate corporate social responsibility?
• What role can HR take in creating a culture of corporate social responsibility?

Use this week’s lecture as a basis for your post. Reference and cite the textbook in your original post

Groups can either become cohesive and harmonious across a life span or can face some relational tensions that can break them up. Relational tensions can include personality issues, emotional conflicts, and people-oriented challenges that the groups face.

In this assignment, you will conduct research using the Argosy University online library resources and the Internet to explore how groups bond together and why these groups last longer than other groups. You will also explore the reasons for relational tensions and how such tensions can break the groups up.

Tasks:

In support of your research, in a minimum of 400 words, respond to the following points:

• Describe a group that you have been a member of for many years. This could be personal or professional.
• Describe the bonds that have been formed in your group.
• State whether the group is still together, and explain the reasons for its current status.
• Describe the relational tensions the group has incurred along the way. What are the implications of these tensions on the group dynamics? How have these tensions been or could be resolved?
• Discuss the manner in which you might avoid issues such as conformity, normative influence, informational social influence, and obedience or any other type of interference in a group supervision

Segmentation and targeting principles are often mistaken as being the same in marketing. For this discussion:

Requirements: at least 470 words and APA references.

• • Define the two concepts of segmentation and targeting.
  • Using a product of your choice, identify a primary customer group based on common, shared characteristics.
  • Include demographic and behavioral characteristics of the customer group.
  • Explain how the product you have chosen would be positioned or repositioned amongst those target customers.